Objectives　Anti-allergic effects of SBN-024, a nasal spray containing beclometasone dipropionate（BDP）as an effective ingredient, were assessed in comparison with Aldecin®AQ nasal and Beconase® using IgE associated nasal-vascular permeability increasing model of rats.
Methods　Passively sensitized rats were prepared by intravenous injection of anti DNP-As rat serum. After the nasal cavity was washed with saline through the polyethylene tube placed in the nasal cavity from the trachea, 4％ pontamine sky blue solution was injected intravenously. The nasal cavity was successively washed and the washed out fluid was collected for 10 min（perfusate before evocation）. Then, each test substance was applied into the nasal cavity, and 30 min later, 2 mg/mL DNP-As was infused in the nasal cavity and placed for 5 min. After that, the nasal cavity was re-washed with saline and washed out fluid was collected for 10 min（perfusate after evocation）. Concentration of dye in the perfusate was determined photometrically at the wave length of 616 nm.
Results　SBN-024（BDP 50 and 25μg/spout）showed significant decrease in the concentration of dye in the perfusate compared to negative controls, and demonstrated similar suppressive effects as Aldecin®AQ nasal and Beconase®. In addition, SBN-024 containing lower doses（BDP 25 and 12.5μg/spout）either showed significant decreases in the concentration of dye in the perfusate compared to negative controls, but the anti-allergic effects were significantly inferior to SBN-024（BDP 50μg/spout）.
Conclusions　It is suggested that SBN-024（BDP 50 and 25μg/spout）has potent therapeutic effects on allergic rhinitis as equally with Aldecin®AQ nasal and Beconase®, and similar effects may be expected for the lower-dosed SBN-024 formulations（BDP 12.5μg/spout）.